By Robert Levenson, Janet Rettig Emanuel, Susan Garetz (auth.), Steve Heinemann, James Patrick (eds.)
This booklet is a set of papers describing a few of the first makes an attempt to use the innovations of recombinant DNA and molecular biology to stories of the apprehensive process. We think this is often a big new course for mind examine that may ultimately result in insights now not pos sible with extra conventional techniques. first and foremost look, the wedding of molecular biology to mind learn turns out an not likely one end result of the super disparity within the histories of those disciplines and the issues they face. Molecular biology is by way of nature a reductionist method of biology. Molecular biologists have continually attempted to assault important questions within the so much direct process attainable, often within the most elementary approach to be had: a bacterium or a bacterial virus. very important experiments can often be repeated quick and affordably, in lots of instances through the most recent workforce of graduate scholars getting into the sector. The luck of molecular biology has been so profound as the results of every one very important scan has made the following severe query visible, and typically answerable, briefly order. stories of the anxious procedure have a truly diverse background. First, the human mind is what relatively pursuits us and it's the most intricate constitution that we all know in biology. The relevant query is apparent: How can we perform better capabilities akin to studying and pondering? How ever, at the present there's no largely authorised and testable thought of examine ing and no transparent route to this kind of theory.
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More importantly, it promises to eventually provide an understanding of the detailed molecular events that both trigger and accompany myelination. GREG LEMKE • Molecular Neurobiology Laboratory, The Salk Institute for Biological Studies, La Jolla, California 92037. 21 Greg Lemke 22 2. FORMATION AND STRUCTURE OF THE MYELIN SHEATH Myelin is formed in the central nervous system by oligodendrocytes (derived from the neural tube) (Wood and Bunge, 1984) and in the periphery by Schwann cells (derivatives of the neural crest) (Asbury, 1985).
The intensity of hybridization of these DNA fragments indicated that rb19 sequences were amplified in HeLa C+ cells. Thus, as we observed in the case of rb5, rb19 is amplified and « « z z 0:: 0:: « Z 0:: . 2: 0:: 0:: 0:: )0- e: Q) e: .... l- Q) ... '" -28 5 -18 5 rb19 Figure 9. Expression of the rb19 gene in rat tissues. RNA was prepared from rat kidney, brain, and liver. g of RNA from each tissue was loaded on separate lanes of a 1% formaldehyde-agarose gel. Following electrophoresis the RNA was transferred to a nitrocellulose filter and the filter was reacted with 2 x 107 cpm of radiolabeled rb19 cDNA as probe.
Kies, M. , and Suckling, A. , 1984, Experimental Allergic Encephalomyelitis, Alan R. Liss, New York. Amara, S. , Rosenfeld, M. , Ong, E. , and Evans, R. ) 298:240-244. Genes Encoding the Major Myelin Proteins 39 Anderson, D. , 1983, Mechanisms of integration of de novo-synthesized polypeptides into membranes, Proc. Nat!. Acad. Sci. USA 80:7249-7253. Asbury, A. K, 1975, The biology of Schwann cells, in: Peripheral Neuropathy, Vol. I (P. J. Dyck, P. K Thomas, and E. H. ), pp. 201-212, W. B. Saunders, Philadelphia.
