By Z. Li, R. L. Jarret, J. W. Demski (auth.), Professor Dr. Y. P. S. Bajaj (eds.)
Twenty-seven chapters care for the regeneration of crops from protoplasts and genetic transformation in a number of species of Arachis, Buplerum, Capsella, Dendrobium, Dianthus, Diospyros, Fagopyrum, Festuca, Gentiana, Glycyrrhiza, Gossypium, Hemerocallis, Levisticum, Lonicera, Musa, Physalis, Platanus, Prunus, Saposhikovia, Solanum, Spinacia, Trititrigia, Tulipa, together with end result corresponding to apricot, banana, cranberry, pepino, peach, and plum. those reports replicate the far-reaching implications of protoplast expertise in genetic engineering of crops. they're of specified curiosity to investigate scientists, lecturers and complex scholars within the fields of plant tissue tradition, molecular biology, genetic engineering, plant breeding and common biotechnology.
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Additional resources for Plant Protoplasts and Genetic Engineering VI
Example text
Exp Cell Res 50: 151-158 Gill R, Saxena PK (1992) Direct somatic embryogenesis and regeneration of plants from seedling explants of peanut (Arachis hypogaea): promotive role of thidiazuron. Can J Bot 70: 1186 -1192 Gregory MP, Gregory WC (1979) Exotic germplasm of Arachis L. interspecific hybrids. J Hered 70: 185-193 Gregory WC, Gregory MP, Krapovickas A, Smith BW,. Yarbrough JA (1973) Structures and genetic resources of peanuts. In: Peanut - culture and uses. Am Peanut Res Educ Assoc Inc, Stillwater, pp 47-133 Regeneration of Plants from Protoplasts of Arachis Species 13 Harms CT, Potrykus I (1978) Fractionation of plant protoplast types by iso-osmotic density gradient centrifugation.
For culture, 3-ml aliquots of protoplast suspensions were 36 M. Nakano and M. Mii d e Fig. la-e. Plant regeneration from protoplasts of Dianthus chinensis cv. Gosun-sekichiku. (Nakano and Mii 1992). 1m. 1m. 1m. d Shoot regeneration from protoplast-derived callus I month after transfer to the regeneration medium; bar I cm. eRegenerated plants growing in the greenhouse (flowering stage) 10 months after initiation of the protoplast culture; bar 2 cm dispensed into 6-cm diameter Petri dishes. ) and maintained at 27 °C in the dark for 2 months.
1 % HgCI2 for 4 min. After washing three times with sterile distilled water and drying with sterile filter paper, slices 1-2 mm in thickness were cut with a scalpel and inoculated on modified MS medium containing 2 mg/12,4-D in diffuse light at 25±1 ·C. White and soft (type A) calli formed in 2 weeks and were transferred on the same medium with I mg/I 2,4-D for subculture every 20 days. Two other types of calli arose and proliferated after subculture for 6 months: one (B) was yellow and compact and the other (C) was light yellow, loose, and granular.
