By E. E. Hansen, J. F. Hubstenberger, G. C. Phillips (auth.), Professor Dr. Y. P. S. Bajaj (eds.)
Twenty-seven chapters care for the regeneration of vegetation from protoplasts and genetic transformation in a number of species of Agrostis, Allium, Anthriscus, Asparagus, Avena, Boehmeria, Carthamus, Coffea, Funaria, Geranium, Ginkgo, Gladiolus, Helianthus, Hordeum, Lilium, Lithospermum, Mentha, Panax, Papaver, Passiflora, Petunia, Physocomitrella, Pinus, Poa, Populus, Rubus, Saintpaulia, and Swertia. those experiences mirror the far-reaching implications of protoplast expertise in genetic engineering of crops.
This quantity is of distinct curiosity to complex scholars, lecturers, and study scientists within the box of plant tissue tradition, molecular biology, genetic engineering, plant breeding, and normal plant biotechnology.
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Extra info for Plant Protoplasts and Genetic Engineering VII
Sample text
In: Evans D, Sharp WR, Ammirato PV (eds) Handbook of plant cell culture, vol 4. MacMillan, New York, pp 419-456 Peffley EB, Mangum PD (1990) Introgression of Allium fistulosum L. : cytogenetic evidence. Theor Appl Genet 79: 113-118 Phillips GC, Hubstenberger JF (1987) Plant regeneration in vitro of selected Allium species and interspecific hybrids. HortScience 22: 124-125 Phillips GC, Luteyn KJ (1983) Effects ofpicloram and other auxins on onion tissue cultures. J Am Soc Hortic Sci 108: 948-953 Rabinowitch HD, Brewster JL (eds) (1990) Onions and allied crops, vol II, Agronomy, biotic interactions, pathology, and crop protection.
Ball (1959) studied the growth of mature embryos in the presence of various carbohydrates. The growth of immature embryos was stimulated with coconut milk, glutamine (Wang and Shen 1965), casein hydrolysate alone or in association with sucrose at high levels (Le Page-Degivry 1967). In vitro cultures were equally initiated from haploid explants. Pollen germination was observed on simple carbohydrate solutions (Kuhlwein 1937), on media supplemented with ovule extracts (Zhukovskii and Medvedev 1949), or complex and modified White (1943) or Tulecke (1953, 1957) media.
5 ml medium in 6-cm Petri dishes. 31 M glucose. Five weeks later, the cultures were transferred to medium without glucose and with 20 gjl sucrose. The medium was replaced with fresh medium after another 4 weeks. For the fifth subculture, calli were transferred onto medium solidified with 2 gjl Gelrite (Kelco, USA) and without growth regulators. This resulted in the formation of globular embryos larger than 300 11m. 26 C. 3 Regeneration of Plantlets Calli with embryos that had developed 7 weeks after the beginning of the fifth subculture were transferred to the same medium as before, but without casein hydrolysate and L-cysteine HCI.
