By D.-P. Häder (auth.), J. Rozema, W. W. C. Gieskes, S. C. Van De Geijn, C. Nolan, H. De Boois (eds.)
Current phase-out schedules of the construction and emission of CFC's point out that chlorine loading within the stratosphere isn't really but at its greatest. The restoration of stratospheric ozone is expected to take time and ele vated degrees of UV-B radiation are anticipated to happen all through lots of the subsequent century. regardless of various physiological experiences of UV-B results on crops, frequently grown in weather chambers, wisdom of UV-B results on organisms and tactics in common aquatic or terrestrial ecosystems is bad. at the moment it seems that UV-B radiation is not only an environmental pressure' issue to crops. In a number of methods, that are incompletely understood, UV-B impacts a variety of physiological and ecological methods. Remarkably, fresh box reports point out that more desirable UV-B doesn't markedly impact photosynthesis, development and first construction, yet relatively interferes with plant morphogenesis and plant and atmosphere features with regards to the secondary metabolism. This distinct factor and booklet UV-B and Biosphere is an try to hide this diversity and to record the development made within the study of ecological results of more suitable sunlight UV-B radiation. The papers during this publication shaped the foundation of a global workshop entitled' UV-B and Biosphere' , December 15-18, 1995, in Wageningen, The Netherlands. a primary response of Hans de Boois at the variety of papers and periods scheduled from Friday to Sunday morning used to be: a ways too many.
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Example text
The initial slope was estimated by linear regression of the first 5 data points. Instantaneous 02-production rates were measured by transferring the algae to the incubator within 1 min. In this case the algae were not dark adapted and were directly exposed to the calculated mean light irradiance inside the culture at the moment of sampling. The short incubation time ofless than 10 minutes prevented supersaturation of 02 and obviated the need for N2 bubbling. Results Under nutrient replete conditions exposure to a low and high UV-B dose inhibited the growth rate of S.
Algae were filtered onto a 2 cm glass fibre filter (Whatman GFfF) and homogenised in 5 ml 90% acetone for 1 min using 12 g of 1 mm glass beads. Chlorophyll a concentrations were calculated according to Jeffrey & Humphrey (1975). Carbohydrate was determined with the anthrone reagent according to Hassid & Abraham (1957). Protein was determined according to Herbert et al. (1971). Bovine serum albumin was used as a standard. Prewashed 2 cm glass fibre filters (Whatman GFfF) dried at 80°C for 6 h were used for dry weight measurements.
1991) is characterised by a computer-controlled dynamic light system and flat geometry of the culture vessel. Besides a brief description of the main characteristics of the newly developed system, a first series of experiments, directed to establish the differences in effects caused by acute and chronic exposure to UV-B radiation is presented here. Additionally, we attempted to identify the primary tar- gets ofUV-B radiation. To investigate the influence of physiological conditions of the algae on their sensitivity to UV-B radiation, experiments were performed under both nutrient-replete and phosphorus-limiting conditions.
