By Jonathan Y. Richmond, Robert W. McKinney
Describes the combos of normal & targeted microbiological practices, defense equip., & amenities that are instructed for paintings with a number of infectious brokers in numerous labs. This revision takes under consideration such matters as international quandary approximately rising & reemerging infectious ailments; elevated curiosity in prion illnesses with the identity of BSE in England; the prevalence of a number of lab-associated infections; elevated predicament concerning the nationwide & intl. move of infectious microorganisms; & becoming issues approximately bioterrorism, & the elevated protection wishes of our microbiological labs.
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Extra resources for Biosafety in Microbiological and Biomedical Laboratories
Broken glassware must not be handled directly by hand, but must be removed by mechanical means such a s a brus h and du stpan, ton gs, or forc eps. Containers of contaminated needles, sharp equipment, and brok en glass are decontam inated before disposal, according to any local, state, or federal regulations. Cultu res, tis sue s, sp ecim ens of bo dy fluid s, or p oten tially infectious wastes are placed in a container with a cover that prevents leakage during collection, handling, proces sing, stora ge, trans port, or sh ipping.
Mouth pipetting is prohibited; mechanical pipetting devices are u sed. 5. Policies for the safe handling of sharps are instituted. 6. All procedures are performed carefully to minimize the creation of aerosols. 7. Work surfaces are decontaminated at least once a day and after any sp ill of viab le m ateria l. 8. All cultures, stocks, and other regulated wastes are decontaminated before disposal by an approved decontamination method, such as autoclaving. Materials to be decontam inated outside of the imme diate laboratory are placed in a durable, leakproof container and closed for transport from the laboratory.
7. , autoclave, chemical disinfection, incineration, or other approved decontamination method). Consideration should be given to means of decontaminating equipment. If waste is transported out of the laboratory, it should be properly sealed and not transported in public corridors. 8. Biological safety cabinets are required and are located away from doors, from room supply louvers, and from heavily-traveled laboratory areas. 9. A du cted exha ust a ir ven tilation syste m is prov ided. This system creates directiona l airflow which draws a ir into the la bora tory fro m "c lean" area s and towa rd "co ntam inated" areas.