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A suite of with ease reproducible experimental equipment for learning the subversion of ordinary mobile signaling that results in melanoma. provided in step by step element to make sure profitable effects, the equipment comprise confirmed thoughts for the research of apoptosis and phone loss of life, complementary protocols for manipulating and/or tracking oncogenic indications in melanoma cells, and strategies for learning protein-protein interactions. a close protocol for shooting natural samples of malignant cells from frozen tissue specimens and replacement options for studying their genomic DNA can be defined.
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Extra info for Cancer Cell Signaling: Methods and Protocols (Methods in Molecular Biology Vol 218)
In the case of Rb−/− prostate epithelium, a cytokeratin profile can be performed using antibodies directed against cytokeratins 14 and 8 as described for fixed tissue in Subheading 3. Immunohistochemistry is an efficient application of these antibodies in cultured cells, allowing for both the evaluation of specific cytokeratins coupled to morphologic profile. As shown in Fig. 6A,B, the morphology of the Rb−/− cultures suggests a mixed epithelial phenotype. Using cytokeratin-specific antibodies in conjunction with cell morphology revealed large, flattened cells that were strongly positive for cytokeratin 14 expression, suggestive of a basal epithelial phenotype (see Fig.
28. Ittmann, M. M. and Wieczorek, R. (1996) Alterations of the retinoblastoma gene in clinically localized, stage B prostate adenocarcinomas. Hum. Pathol. 27, 28–34. 29. Brooks, J. , Bova, G. , and Isaacs, W. B. (1995) Allelic loss of the retinoblastoma gene in primary human prostatic adenocarcinomas. Prostate 26, 35–39. 30. Bookstein, R. (1994) Tumor suppressor genes in prostatic oncogenesis. J. Cell. Biochem. ), 217–223. 31. Vesalainen, S. and Lipponen, P. (1995) Expression of retinoblastoma gene (Rb) protein in T12M0 prostatic adenocarcinoma.
1981) Establishment in culture of pluripotential cells from mouse embryos. Nature 292, 154–156. 2. Martin, G. R. (1981) Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells. Proc. Natl. Acad. Sci. USA 78, 7634–7638. 3. Koller, B. , Hageman, L. , Doetschman, T. , Hagaman, J. , Williams, P. , et al. (1989) Germline transmission of a planned alteration made in the hypoxanthine phosphoribosyltransferase gene by homologous recombination in embryonic stem cells.