Read e-book online Cryopreservation of Plant Germplasm I PDF

By Y. P. S. Bajaj (auth.), Y. P. S. Bajaj (eds.)

Whole crops were regenerated from remoted protoplasts, cells, tissues, and organs freeze-preserved in liquid nitrogen. hence cryopreservation has proved to be a huge instrument for the garage and conservation of plant genetic assets.
Numerous specialists have contributed to this quantity, that's divided into the next sections: I Cryopreservation of Plant Cells, Tissues, Organs, and Seeds; II Tree species - Citrus, Malus, Morus, Pyrus, Juglans, Date-Palm, Oil Palm, Coconut, Veitchia, Howea, Calamus, Hevea, Larix, Picea, and Pinus; III Cereals and Grasses - Wheat, Rice, Maize, Setaria, Puccinellia, and Sugarcane; IV Legumes and Oilseed vegetation - Arachia hypogaea, Cicerarietinum, Pisum sativum, Medicago, Trifolium and Brassica

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Sample text

Complete vitrification eliminates concern for the potentially damaging effects of intra- and extracellular crystallization. Vitrification, in fact, is the formation of a glass-like, noncrystalline solid at the freezing point of an aqueous solution. At sufficiently low temperatures, a highly concentrated cryoprotective solution becomes so viscous that it solidifies into a metastable glass. The goal is to achieve a sufficiently high concentration of solutes to prevent the solution from freezing into crystalline ice when cooled and to ensure its transition into the amorphous or glassy state.

Hokkaido Nat Agric Stn Res Bull 136: 15-21 Laine E, Bade F, David A (1992) Recovery of plants from cryopreserved embryogenic cell suspensions of Pinus caribaea. Plant Cell Rep II: 295-298 Langis R, Schnabel B, Earle ED, Steponkus PL, (1990a) Cryopreservation of Brassica campesteris L. cell suspensions by vitrification. Cryo-Lett 10: 421-428 Cryopreservation of Plant Cell, Tissue, and Organ Culture 25 Langis R, Schnabel B, Preikstas B, Earle ED, Steponkus PL (1990b) Cryopreservation of carnation shoot tips by vitrification.

Euphytica 28: 267-285 Bajaj YPS (1979b) Establishment of germplasm banks through freeze storage of plant tissue cultures and their implications in agriculture. In: Sharp WR, Larsen PO, Paddock PO, Raghavan V (eds) Plant cell and tissue culture - principles and applications. Ohio State Univ Press, Columbus, pp 745-774 Bajaj YPS (I 979c) Freeze preservation ofmeristems of Arachis hypogaea and Cieer arietinum. Indian J Exp Bioi 17: 1405-1407 Bajaj YPS (l980a) Freeze preservation of plant cells - a novel approach to the conservation of germplasm.

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