By W. J. Pledger, J. E. Estes, P. H. Howe, E. B. Leof (auth.), Jennie P. Mather (eds.)
The merits of acquiring a very outlined setting for the expansion of cells in vitro have been well-known very early within the historical past of mobile tradition (Lewis and Lewis, 1911). endured curiosity within the dietary requisites of cells in vitro and in delivering an optimum atmosphere for cells ended in the improvement of the advanced nutrient combos to be had at the present time in lots of media (Waymouth, 1972; Ham, 1965). although, serum remained an integral part of medium for the expansion of so much mobile kinds in tradition. The query of what issue (or elements) in serum was once crucial for phone progress and survival remained unanswered for a number of a long time. first and foremost, experiments have been designed to purify the "active part" of serum for the expansion of cells in tradition. those experiments pointed out fetuin (Fisher et at., 1958) and nonsuppressible insulinlike job (Temin et at., 1972) as vital parts of serum. in spite of the fact that, the complexity of serum and the very low degrees of lively parts in serum hindered development in identi fying and setting apart serum factors.
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Additional info for Mammalian Cell Culture: The Use of Serum-Free Hormone-Supplemented Media
Sample text
Hemoglobin transports oxygen, albumin binds a wide range of essential compounds such as steroid hormones or vitamins, and transferrin binds iron and facilitates its transport into cells. Transferrin and iron are essential for growth of cells in culture as well as in vivo. , 1979; Barnes and Sato, 1980, for examples). Transferrin facilitates iron transport throughout the cell cycle, but does not regulate growth. , 1979). , 1980). In contrast, transferrin and iron deprivation resulted in cell death or growth arrest throughout the cell cycle.
Cherington transformed rat kidney cells. , 1980), thus conferring a transformed phenotype on normal cells. Secretion of TGFs is not evident in all transformed cells (Cherington and Pardee, 1982). Either some transformed cells do not secrete these endogenous growth factors or they secrete only sufficient quantities to stimulate the cell producing them. Concentrations reached in the medium may not be sufficient to stimulate neighboring cells or cells in heterologous cultures. Replacing Serum with Defined Medium Components to Study Growth Regulation in Vitro The use of serum-free or serum-depleted conditions to study factors that stimulate DNA synthesis in growth-arrested cells predates our ability to grow and passage cell lines in the absence of serum.
1965). What originally made this model of growth control particularly interesting were demonstrations that agents that induced tumors in vivo also induced overgrowth of quiescent cell monolayers into piles of cells called foci. Early demonstrations of in vitro transformation included studies of carcinogenic hydrocarbons (Chen and Heidelberger, 1969), Rous sarcoma virus (Temin and Rubin, 1958), and the DNA! tumor viruses SV40 (Todaro and Green, 1964), and polyoma (Stoker and MacPherson, 1961).
