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Eppendorf thermomixers contain 24 spaces, so 24 conditions at one temperature is the maximum you could try. If you have more than one shaker, it is convenient to test several temperatures in parallel. A manageable setup is a total of 24 cultures split between two temperatures 30 Shaw and Miroux Table 1 Optimization of Growth Conditions of the E. 6 Induce at OD600 = 1 (room temperature [RT] and 37°C or RT and 15°C, for example). Two different constructs can be tested for a range of inducer concentrations and also timing and length of induction.
Chem. 277:10,949–10,959. Schöpfer, R. (1993) The pRSET family of T7 promoter expression vectors for Escherichia coli. Gene 124:83–85. , Schlitt, A. Scheide, D. Guenebaut, V. and Friedrich T. (1999) Overexpression of the Escherichia coli nuo-operon and isolation of the overproduced NADH:ubiquinone oxidoreductase (complex I). Biochemistry 38:16,261–16,267. Studier, F. W. (1991) Use of bacteriophage T7 lysozyme to improve an inducible T7 expression system. J. Mol. Biol. 219:37–44. , and Grisshammer R.
Studier, F. , Rosenberg, A. , Dunn, J. , and Dubendorff, J. W. (1990) Use of T7 RNA polymerase to direct expression of cloned genes. Methods Enzymol 185, 60–89. , Hawkins, M. and Weeds, A. G. (1990) Identification of a region in segment 1 of gelsolin critical for actin binding. Embo J. 9, 4103–9. , Nilsson, L. & Kurland, C. G. (1995) Gratuitous overexpression of genes in Escherichia coli leads to growth inhibition and ribosome destruction. J. Bacteriol. 177, 1497–1504. Walker, J. E. and Miroux, B.
