By Hideharu Anazawa, Sakayu Shimizu
Microbial creation: From genome layout to mobile floor engineering provides a finished evaluation of novel expertise and methods being carried out for production microorganisms, written through experts in either academia and undefined. This ebook is split into 3 sections: the 1st contains expertise for development of fermentation traces and plenty of helping applied sciences and data; the second one examines novel know-how worthy for research of mobilephone actions, studying gene functionality, and designing genomes of manufacturer traces; and at last, a dialogue of the sensible software of the thoughts and luck case reports in lots of fields of bio-production, equivalent to microbiological creation, prescribed drugs, chemical substances, meals and cosmetics.
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Extra info for Microbial Production: From Genome Design to Cell Engineering
Example text
These results indicate elimination of toxic genes and unnecessary genes is a useful and powerful concept for designing a mutant strain for microbiological production as a breeding host strain. However, there are some cases showing higher productivity than MGF-01 in a mutant strain on the way to breeding MGF-01 for some other products. In such cases, the deleted region in the next operation step would be restored to the mutant and productivity could be recovered. Thus, there are some effective genes in the region of deletion for the product and this gene becomes a candidate for gene amplification for production.
1 H. Kumagai et al. Schizosaccharomyces pombe New pathways for designing microorganisms for industrial-scale production of biological materials have been made possible by significant advances in genome sequencing, bioinformatics, and genetic engineering. The so-called minimum genome factory (MGF) has been created in which unnecessary or detrimental genes are deleted from the microorganism, leaving only the genes necessary for industrial production of the desired molecule. The first examples of MGFs are Escherichia coli, Bacillus subtilis, and Schizosaccharomyces pombe (S.
Mol Microbiol 65:642–654 Fabret C et al (2002) A new mutation delivery system for genome-scale approaches in Bacillus subtilis. Mol Microbiol 46:25–36 14 K. Ara et al. Hakamada Y et al (2000) Deduced amino acid sequence and possible catalytic residues of a thermostable, alkaline cellulase from an alkaliphilic Bacillus strain. Biosci Biotechnol Biochem 64:2281–2289 Kageyama Y et al (2009) Genome reduction in Bacillus subtilis and enhanced productivities of recombinant proteins. In: Knudsen WD, Bruns SS (eds) Bacterial DNA, DNA polymerase and DNA helicases.
