Download e-book for kindle: Analytical Biotechnology by C. van Dijk (Eds.)

By C. van Dijk (Eds.)

ANABIOTEC '92 inquisitive about the extra integration of biotechnology and analytical chemistry. the result of this symposium in actual fact established mammoth development might be stated within the software of either traditional and new analytical ideas, the latter basically according to ordinary analytical instruments corresponding to biomolecules. the most issues lined in this assembly are fermentation tracking, chromatography, instrumental research, biosensors and bioanalysis

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Where / is the flux (m 3 s _ 1 ), Δ Ρ the pressure applied across the membrane (bar), η the filtrate viscosity (kg m s - 1 ) and Rtot the total hydraulic resistance of the filtration system ( m - 5 ) , which consists of Rm, the resistance of the membrane itself, and Rcp, the resistance of the concentration polarization layer, which is formed on the membrane as a result of the accumulation of retained solutes. Rm is given by: RESULTS AND DISCUSSION Filtration For the appropriate monitoring of bioprocesses the delay time between sampling and presentation of the data should be short compared with the kinetics of the fermentation.

O. A. Marko-Varga, Anal. , submitted for publication. C. van de Merbel, H. Th. Brinkman Department of Analytical Chemistry, Free University, De Boelelaan 1083, 1081 HVAmsterdam (Netherlands) A. C. de Rijke Pharmacia LKB Biotechnologie Nederland, Houttuinlaan 4, 3447 GM Woerden (Netherlands) (Received 1st October 1992; revised manuscript received 15th January 1993) Abstract The efficient control of fermentation processes requires reliable monitoring systems. This paper describes a fully automated monitoring system based on the on-line combination of ultrafiltration and column liquid chromatography.

0 mM. Detector, refractive index (attenuation 16); injection volume, 20 μΐ. For further details, see text. 115% for arabinose (samples taken after 25 min). As the cellulose-based membrane may not be so chemically inert as the polymer-based membrane, this type of membrane was immersed in a 10% TABLE 3 Recovery of xylose and arabinose in a model solution '' Recovery calculated from Recovery (%) Xylose Arabinose Area: 25 min 50 min 100 min 168 100 110 99 109 118 Height: 25 min 50 min 100 min 115 96 106 122 103 112 a Solution C, representing a solution containing interfering compounds.

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