By Gary D. Christian
Widely revised and up-to-date with a extra glossy taste and a brand new, two-color layout, this 6th variation offers with rules and methods of quantitative research. Examples of analytical strategies are drawn from such parts as existence sciences, scientific chemistry, air and water toxins, and commercial analyses. New to this variation: Excel spreadsheets on CD-ROM * New chapters on sturdy laboratory perform, in addition to genomics and proteomics * A extra sleek style.
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Extra resources for Analytical Chemistry
Sample text
4 Confidence limits of X = (Y - A ) / B for given signal value Y K 2 C = B2 - (t • sR ; ) = auxiliary term K In Figure 1, the confidence interval for X 3 is shown, with limits X{ and X 2 . 5) in equation IV: Equation IV is directly deduced from equation II. This, for describing Figure 1, is taking the form: C v C V \ n K Substituting YUP for Yo results in X 2 being identical with LDC, and one obtains (IV). 9 Determination of SY SY is identical with the product of sR and the square root expression in equation I (cf.
5: Statistical analysis of instrument response, J. Assoc. Off. Anal. Chem. 61, 1404-1414 (1978). L. P. M. Weppelman and H. Mehta, Determining the lowest limit of reliable assay measurement, Anal. Chem. 55, 638-643 (1983). H. -P. Thier, Die Ermittlung der Nachweisgrenze und Bestimmungsgrenze bei Ruckstandsanalysen nach dem neuen DFG-Konzept, GIT Fachzeitschrift fur das Laboratorium 35, 285-291 (1991). -H. Holtz; in collaboration with H. Frehse, S. -P. Thier and H. Frehse 9 Examples In this chapter, examples will be given on how to derive the LDC and LDM from a series of measurements.
Without computer support, it is rather laborious to construct the upper and lower prediction limits. In general, however, these limits are only needed in a region between X = 0 and X = X 2 (see Figure 1). For a graphical derivation of the LDC and LDM it is, therefore, permissible to substitute the curves for these limits by straight lines, which are drawn, parallel to the calibration line, through the points YUP and Y ^ . ) This simplification permits the derivation of the LDC and LDM "by hand" on graph paper.
