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By Christian W. Klampfl, Markus Himmelsbach (auth.), Nicola Volpi (eds.)

Simple carbohydrates, complicated oligosaccharides and polysaccharides all belong to a category of ubiquitous (macro)molecules that express a variety of organic features, and the hot introduction of more suitable enzymatic, chemical and analytical instruments used to review those sugars has inaugurated a real explosion within the box of glycomics. in particular, it has resulted in a deeper realizing of the way particular sugar constructions modulate mobile phenotypes, and that step forward has resulted in the invention of latest prescription drugs for the therapy of many severe illnesses, akin to melanoma. the following quick enlargement of this study holds excessive promise for destiny healing regimens, and capillary electrophoresis (CE) refers back to the variety of comparable separation recommendations which are essential to this very important examine. CE makes use of narrow-bore fused-silica capillaries to split a fancy array of huge and small molecules, and Capillary Electrophoresis of Carbohydrates deals a finished examine the newest breakthroughs and enhancements in CE and CE strategies utilized to monosaccharides as much as advanced oligosaccharides and polysaccharides. It starts with an summary of the appliance of CE and CE- mass spectrometric within the research of straightforward carbohydrates with none past derivatization step earlier than discussing quite a few detection suggestions resembling spectrophotometric detection, electrochemical detection and different much less universal options. It then covers intimately an array of comparable subject matters and various purposes. it's a necessary textual content for somebody exploring the myriad chances of this swiftly increasing field.

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Extra info for Capillary Electrophoresis of Carbohydrates: From Monosaccharides to Complex Polysaccharides

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Soga T, Imaizumi M (2001) Capillary electrophoresis method for the analysis of inorganic anions, organic acids, amino acids, nucleotides, carbohydrates and other anionic compounds. Electrophoresis 22:3418–3425. 22. Soga T, Serwe M (2000) Determination of carbohydrates in food samples by capillary electrophoresis with indirect UV detection. Food Chem 69:339–344. 23. Soga T, Ross GA (1999) Simultaneous determination of in organic anions, organic acids, amino acids and carbohydrates by capillary electrophoresis.

Baldwin RP (1999) Electrochemical determination of carbohydrates: enzyme electrodes and amperometric detection in liquid chromatography and capillary electrophoresis. J Pharm Biomed Anal 19:69–81. 45. Zemann AJ (2001) Conductivity detection in capillary electrophoresis. TRAC-Trends Anal Chem 20:346–354. 46. Zemann AJ, Schnell E, Bonn GK (1998) Contactless conductivity detection for capillary electrophoresis. Anal Chem 70:563–567. 47. Fracassi da Silva JA, do Lago CL (1998) An oscillometric detector for capillary electrophoresis.

Kölhed M, Karlberg B (2005) Capillary electrophoretic separation of sugars in fruit juices using on-line mid infrared Fourier transform detection. Analyst 130:772–778. 77. Ivanov AR, Nazimov IV, Lobazov AP, Popkovich GB (2000) Direct determination of amino acids and carbohydrates by high-performance capillary electrophoresis with refractometric detection. J Chromatogr A 894:253–257. 78. Swinney K, Pennington J, Bornhop DJ (1999) Universal detection in capillary electrophoresis with a micro-interferometric backscatter detector.

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