By Bernard Valeur
This present day, fluorescence spectroscopy is a crucial software of research in lots of components. In analytical sciences, its virtue is very excessive sensitivity and selectivity - even unmarried molecules may be detected - and it achieves a excessive spatial solution and time solution together with microscopic recommendations or laser strategies, respectively. In fabric sciences, this can be used to check constitution and dynamics of surfaces. relatively within the parts of biochemistry and molecular genetics, fluorescence spectroscopy has develop into a dominating strategy. including the newest imaging innovations, fluorescence spectroscopy permits a real-time remark of the dynamics of intact organic platforms with an remarkable resolution.
This ebook bargains a accomplished creation to and survey of fluorescence spectroscopy. it's written for beginners and energetic researchers alike who're studying to use fluorescence tools within the parts of chemistry, actual chemistry, polymers, fabrics, colloids, biochemistry, biology, scientific and pharmaceutical examine.
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Additional resources for Molecular Fluorescence: Principles and Applications
Sample text
Eds) (2001) New Trends in Fluorescence Spectroscopy. Applications to Chemical and Life Sciences, Springer-Verlag, Berlin. Wolfbeis O. S. ) (1993) Fluorescence Spectroscopy. New Methods and Applications, Springer-Verlag, Berlin. 19 Molecular Fluorescence: Principles and Applications. Bernard Valeur > 2001 Wiley-VCH Verlag GmbH ISBNs: 3-527-29919-X (Hardcover); 3-527-60024-8 (Electronic) 20 2 Absorption of UV–visible light La lumie`re (. ) donne la couleur et l’e´clat a` toutes les productions de la nature et de l’art; elle multiplie l’univers en le peignant dans les yeux de tout ce qui respire.
Dept. Commerce, New York. Miller J. N. ) (1981) Standards in Fluorescence Spectrometry, Ultraviolet Spectrometry Group, Chappman and Hall, London. Murov S. , Carmichael I. and Hug G. L. (1993) Handbook of Photochemistry, 2nd edn, Marcel Dekker, New York. O’Connor D. V. and Phillips D. (1984) TimeCorrelated Single Photon Counting, Academic Press, London. Parker C. A. (1968) Photoluminescence of Solutions, Elsevier, Amsterdam. Pesce A. -G. and Pasby T. L. (Eds) (1971) Fluorescence Spectroscopy, Marcel Dekker, New York.
According to IUPAC recommendations (see Pure & Appl. Chem. e. the radiant power at wavelength l per unit wavelength interval. Radiant power is synonymous with radiant (energy) flux. The SI unit for radiant power is J sÀ1 ¼ W; the SI unit for spectral radiant power is W mÀ1 , but a commonly used unit is W nmÀ1 . 23 24 2 Absorption of UV–visible light Failure to obey the linear dependence of the absorbance on concentration, according to the Beer–Lambert Law, may be due to aggregate formation at high concentrations or to the presence of other absorbing species.