By Veronika R. Meyer
Veronika Meyer’s booklet on HPLC is a vintage textual content and continues to be one of many few titles to be had on common HPLC. Following on from the luck of the former 3 variations, this new, fourth variation keeps to supply clients of HPLC in undefined, executive, and repair laboratories, in addition to postgraduate scholars, with a unified method of HPLC and an equivalent remedy of the speculation and perform of this significant procedure.
The contents of this version were revised, elevated and up to date. the place on hand, previous literature references were changed via contemporary ones. New sections at the following subject matters were included:
- Shelf-live of cellular stages
- The blending pass
- Phase structures in ion chromatography
- Measurement uncertainty
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Additional resources for Practical high-performance liquid chromatography
Sample text
S. Jeansonne and J. P. Foley, J. Chromatogr. , 29, 258 (1991). 8 PEAK CAPACITY AND STATISTICAL RESOLUTION PROBABILITY17 The efficiency of a separating system is best demonstrated by its peak capacity. This shows how many components can be separated in theory within a certain k range as peaks of resolution 1. The number of theoretical plates, N, of the column used must be known, as the peak capacity, n, is proportional to its square root:18 pffiffiffiffi N ln ð1 þ k max Þ n¼1þ 4 where k max ¼ maximum k value.
Solution t 0 ¼ 1 min; t R max ¼ 5 min. 5À1 ¼4 1 pffiffiffiffiffiffiffiffiffiffiffiffiffi 10 000 n¼1þ ln ð1 þ 4Þ ¼ 1 þ ð25 Â 1:61Þ 4 n ¼ 41 k max ¼ The above-mentioned equation for n in fact is only valid for isocratic separations and if the peaks are symmetric; the peak capacity is larger with gradient separations. Tailing decreases the peak capacity of a column. In real separations the theoretical plate number is not constant over the full k range. However, it is even more important to realize that a hypothetical parameter is discussed here.
Those two components of the mixture that are most difficult to separate. 25 —————————— 25 R. P. W. Scott, J. , 468, 99 (1989). 1 GENERAL REQUIREMENTS An HPLC pump unifies two different features: It must be a sturdy device capable of generating high pressure up to 350 or even 500 bar; conjointly the user demands high flow accuracy and precision at any flow rate which is chosen. 1 ml min À1 . The delivered flow rate must be independent of the back pressure, even if this changes during a separation, which is usually the case with gradient elution.
