By S. A. Abrams, W. W. Wong
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Additional info for Stable Isotopes in Human Nutrition: Laboratory Methods and Research Applications (Life Sciences)
Example text
The similarity of these findings suggests that the VLDL-apoB-100bound tracer enrichment at plateau is the same as the hepatic t-RNA enrichments when the tracer is given by a prime-constant intravenous infusion. We have now adopted this approach in all of our human studies. , 1992). , 1994). We found that both plasma alanine and pyruvate were similarly enriched as apoB-bound alanine. , 1994). A disadvantage of this approach is that both uniformly labelled glucose and alanine are expensive. A Practical Example: Prealbumin and VLDL-apoB-100 Synthesis Because we use the steady-state isotopic enrichment of VLDL-apoB-100 to estimate the isotopic enrichment of the hepatic precursor pool in all of our studies, the fractional rate of synthesis of VLDL-apoB-100 can also be obtained in any study performed to measure the rate of synthesis of a particular hepatic-derived plasma protein.
One catheter is used for infusion of isotope and the other for blood sampling. 5 g l–1 saline and infused continuously for 8 h at 10 µmol kg–1 h–1 through the catheter in one forearm after a priming dose of 9 µmol kg–1 is injected. A 4 ml blood sample is drawn before the start of the infusion, followed by additional blood samples at 1 h intervals for the rest of the infusion. Sample analyses Blood is drawn in pre-chilled tubes containing 10 µl of a solution consisting of 10% Na2EDTA, 2% sodium azide, 1% merthiolate and 2% soybean trypsin inhibitor, immediately centrifuged at 2000 × g for 15 min at 4oC.
And de Benoist, B. (1987) Whole-body protein turnover in man determined in three hours with oral or intravenous 15N-glycine and enrichment in urinary ammonia. Human Nutrition Clinical Nutrition 41, 263–276. J. E. (1994) Measurement of plasma protein synthesis rate in the infant pig: an investigation of alternative tracer approaches. American Journal of Physiology 267, R221–R227. , Frazer, E. J. (1999a) Chronic protein undernutrition and an acute inflammatory stimulus elicit different protein kinetic responses in plasma but not in muscle of young pigs.



