By Hassan Y. Aboul-Enein
Experiences up to date examine advancements on purifying and isolation huge natural molecules. The textual content presents details on high-performance liquid chromatography and capillary electrophoresis (CE) as instruments for interpreting biomacromolecules and constructing new biochemical and medicinal compounds. It applies biochemical separation expertise to the examine of macromolecules corresponding to proteins, polysaccharides, nucleic acids and extra.
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Additional info for Analytical and Preparative Separation Methods of Biomacromolecules
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4), which had the same amino acid compositions as well as the same molecular mass of approximately 680 Da. Isomerization of the N-terminal peptide fragment obtained after tryptic digestion of rSmp2812 can be explained by the fact that the peptide contains a cyclic linkage that formed during digestion. Since this phenomenon was only observed in the case of rSmp2812, it is likely to be related to the original N-terminal modification that led to the shift in isoelectric point. To test the hypothesis of a cyclic linkage, both the free and blocked Nterminal peptides were subjected to deuterium exchange followed by MS.