By Thorsten Teutenberg
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Additional info for High-Temperature Liquid Chromatography
Sample text
I will explain the necessity for using this technique in Chapter 6, where the focus is on method development strategies. Moreover, I will give examples based on new technologies and methods in Chapter 8, which rely on temperature programming. However, before I deal with applications where temperature programming is a prerequisite, I would like to explain how a temperature gradient works in contrast to a solvent gradient. 12 Chapter 3 Peak area of three PAH at different eluent temperatures. : 1 ml; detection: UV DAD at 254 nm, with a fixed cell temp.
8 Dependence of measured eluent temperature in a stainless steel capillary on different measuring positions at a constant flow rate of 2 ml minÀ1. 5 Temperature was measured at position 4. ) These results serve as an experimental validation and underline that the mathematical calculations made by Abbott can be met experimentally. When the results are compared to the experimental validation of Guillarme et al. 1, it is obvious that contact heating is better suited to the rapid preheating of the mobile phase within a very short distance.
The mathematical treatment as well as the experimental evidence unambiguously suggest that it is not necessary to use an extremely long preheating capillary. If the heating system is well designed, a few centimetres will suffice. Hence, the fear that a preheating coil will significantly add to the extra-column volume is clearly overestimated. Guillarme et al. 7 They calculated the necessary length of the preheating capillary when an air-based oven was used and flow rates between 3 and 10 ml min 1 were applied.



