By H. T. Tien †, A. Ottova-Leitmannova
The lipid bilayer is the main uncomplicated structural component of telephone membranes. a variety of issues are coated during this quantity, from the beginning of the lipid bilayer thought, to present purposes and experimental recommendations. each one bankruptcy during this quantity is self-contained and describes a group's learn, delivering particular method and key references worthy for researchers. Lipid bilayer study is of significant curiosity to many due to it is interdisciplinary nature. ·Provides an outline of a long time of study at the lipid bilayer·38 contributed chapters, by way of prime scientists, hide quite a lot of subject matters in a single authoritative volume·Book coincides with fortieth anniversary of BLM
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Extra info for Planar Lipid Bilayers (BLMs) and Their Applications
Sample text
Muto and colleagues [29] reported that, at cytotoxic concentrations, blepharismins formed cation-selective channels in planar phospholipid bilayers. 6:1. 1 M KC1, possibly reflecting different states of aggregation of blepharismin. The observations of Muto et al suggest that channel formation is a cytotoxic mechanism of blepharismin's action against predatory protozoa. In this connection, the apical brush border membrane, the main target site of Bacillus thuringiensis toxins, was isolated from gypsy moth (Lymantria dispar) larval midguts and fused to planar lipid bilayers, as reported by Peyronnet et al [30].
Jovov and associates [72] then concluded that actin serves an important regulatory function for ENaC and that planar bilayers are an appropriate system in which to study the biophysical and regulatory properties of these cloned channels. The ubiquity of mechanosensitive (MS) channels triggered a search for their functional homologues in Archaea, the third domain of the phylogenetic tree. Two types of MS channels have been identified in the cell membranes of Haloferax volcanii using the patch clamp technique, as reported by Kloda and Martinac [75].
One exception is the plasma membranes from erythrocytes 22 Chapter 1 (RBCs) that can be isolated in high purity because these cells contain no intemal membranes. As discussed recently by Van Dort et al. [21 ], three major hypotheses have been proposed to explain the role of membrane-spanning proteins in maintaining membrane stability. These hypotheses attribute the essential contribution of integral membrane proteins to (i) their ability to anchor the membrane skeleton to the lipid bilayer, (ii) their capacity to bind and stabilize membrane lipids, and (iii) their propensity to influence and regulate local membrane curvature.
