By Zdeněk Deyl
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Additional resources for Separation methods
Example text
The classification of electromigration methods varies according to the criteria used. 1. The comparison of the course of separation as the result of the experimental arrangement is schematically shown in Fig. 1. In the zone electrophoresis method the sample is placed in a definite area of the separation column filled with the electrolyte. After the electrical field has been imposed onto the system, individual particles migrate, according to their effective mobilities with different speeds, towards the respective electrodes (and, concomitantly, both positively and negatively charged particles are separated).
Removal of excess reagent through a double-column arrangement. See text for explanation. Reproduced from [35]. 53 The current techniques in multidimensional chromatography have recently been reviewed [37,38]. Multicolumn GC techniques can undoubtedly be of advantage in numerous analytical situations. Applications of these techniques in biochemical analysis have been thus far limited. Although some commercial units of this kind appeared recently, most investigators work with conventional modified instruments.
Selective retention of n-hydrocarbons on a molecular sieve or olefins on silver nitrate-coated supports are now considered classical examples of this behavior. More recently, Picker and Sievers [34] used a short column loaded with a europium (111) coordination polymer sorbent to trap selectively various oxygenated compounds from a complex mixture of urinary volatile metabolites. Double-column GC systems, utilizing a short packed column and an analytical capillary column with an intermediate trapping system, have been used in a variety of situations: (a) sampling and enrichment of the trace quantities of analytes from a dilute solution; (b) removal of solvents and derivatization reagents for improving performance of capillary columns and certain GC detectors; (c) direct injection of aqueous samples with subsequent removal of the water peak; and (d) backflushing applications.
